How to Use a Compound Light Microscope for Lab Procedures
- 1). Carry the microscope to a flat and sturdy surface, using one hand to hold main shaft from the eye piece to the base and the other hand to hold it from the base.
- 2). Turn on the light source and place the slide or material on the stage. The proper position for the sample is directly over the light source.
- 3). Set the microscope to the lowest objective powered lens and use the coarse and fine focus knobs to bring the image into focus. Always look into the ocular lens with both eyes open, resisting the urge to close one eye and squint with the other which will increase the strain and tension on your eyes.
- 4). Adjust the diaphragm as needed to allow more or reduce the amount of light let in.
- 5). Switch to higher magnification powers as needed, refocusing each time if needed. Some compound microscopes are parfocal, meaning they will remain in focus even if you change magnification.
- 6). Wipe the lenses with lens paper if dirty then cover the microscope once finished.
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